P3 Research Seminar
Assessing Plasma Meropenem Concentrations: Validation of an HPLC Protocol
Document Type
Student Research Paper
Presentation Date
4-13-2015
Abstract
Abstract
Purpose: The purpose of this study was to validate a laboratory protocol for comparing the assessing the concentration of meropenem in plasma samples.
Methods: To validate the laboratory component of a clinical protocol, plasma samples with meropenem and an internal standard of ceftazidime were analyzed using a Dionex® Ultimate 3000 HPLC and both an Acclaim® 120 C18 guard column (4.6 x 10 mm, 5 µm silica), and an Acclaim® 120 C18 analytical column (4.6 x 150 mm, 5 µm silica). Samples analyzed included quality control, calibration standards, and spiked unknowns blinded to the analysts. Each sample was prepared according to protocol and run at ambient temperature with a flow rate of 1 mL/min and an injection volume of 10 µL, in a mobile phase composed of 92% 0.1M mixed phosphate buffer (pH 6.8) and 8% acetonitrile with a stabilizer buffer of 3-[N-morpholino]propanesulfonic acid (MOPS).
Results: After analysis of a calibration curve and spiked samples, the correct concentration (± 10 µg/mL) was determined for nine out of ten plasma samples. However, there was significant variation in the data obtained by individual analysts.
Conclusions: The HPLC method is a valid analytical protocol for determining plasma concentration of meropenem. However, further laboratory training of the analysts is needed before analyzing patient samples.
Keywords
Meropenem, HPLC, cystic fibrosis, pediatric, nausea
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
