Type of Submission
Poster
Keywords
3D Printing, three dimensional printing, scaffold, biodegradable, tissue engineering, cell growth, regenerative medicine, cell culturing
Abstract
With the recent improvements in three dimensional (3D) printing technologies, the potential for tissue engineering and regenerative medicine have significantly improved. One key idea in tissue engineering is to specifically design scaffolds to aid in the healing process by being incorporated into the body’s own tissue. The overall goal of this project is to investigate 3D printable scaffold design to access suitability for tissue replacement. This was accomplished by analyzing the effect of the material used to create the scaffolds, pore size, and pore shape on mechanical stiffness and cell culturability. Based on published literature, it was determined that, depending upon the desired tissue type, the best pore shapes are circles, squares, and hexagons. This study focused on designing numerous scaffolds by varying the parameters listed above, and then printing 3D biodegradable (PLA & TPU) scaffolds to be cultured, mechanically tested and evaluated. The scaffolds were cultured with endothelial cell lines to ensure cell survivability on the 3D printed material. After cell culturing protocol, cell attachment and viability were assessed and cell density recorded. The mechanical tests were performed using a standard tension test machine in order to gather stiffness and strength data. By analyzing our results, we will be able to make recommendations regarding which pore shape, size, and porosity will yield the most anatomically compliant results for the desired tissue.
Faculty Sponsor or Advisor’s Name
Dr. Timothy Norman and Dr. Rocco Rotello
Campus Venue
Stevens Student Center
Location
Cedarville, OH
Start Date
4-12-2017 11:00 AM
End Date
4-12-2017 2:00 PM
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Download file contains only the abstract
Included in
Biology Commons, Biomaterials Commons, Mechanical Engineering Commons, Molecular, Cellular, and Tissue Engineering Commons
3D Printing of Biodegradable Scaffolds for Tissue Engineering Applications
Cedarville, OH
With the recent improvements in three dimensional (3D) printing technologies, the potential for tissue engineering and regenerative medicine have significantly improved. One key idea in tissue engineering is to specifically design scaffolds to aid in the healing process by being incorporated into the body’s own tissue. The overall goal of this project is to investigate 3D printable scaffold design to access suitability for tissue replacement. This was accomplished by analyzing the effect of the material used to create the scaffolds, pore size, and pore shape on mechanical stiffness and cell culturability. Based on published literature, it was determined that, depending upon the desired tissue type, the best pore shapes are circles, squares, and hexagons. This study focused on designing numerous scaffolds by varying the parameters listed above, and then printing 3D biodegradable (PLA & TPU) scaffolds to be cultured, mechanically tested and evaluated. The scaffolds were cultured with endothelial cell lines to ensure cell survivability on the 3D printed material. After cell culturing protocol, cell attachment and viability were assessed and cell density recorded. The mechanical tests were performed using a standard tension test machine in order to gather stiffness and strength data. By analyzing our results, we will be able to make recommendations regarding which pore shape, size, and porosity will yield the most anatomically compliant results for the desired tissue.
