Type of Submission

Poster

Award

Best Experimental Study

Keywords

netrin-4, Tetrahymena, chemorepellent

Abstract

Netrins are signaling proteins, acting as chemorepellants or chemoattractants, and their role is especially important in early growth in organisms. In studies involving Tetrahymena thermophila, netrin proteins often act as chemorepellants, so research centered around verifying if this was also true for Netrin-4 protein. Since Netrin-1 and Netrin-3 have been shown to influence neurological and developmental growth in organisms, the implications for discovering the cellular effects of Netrin-4 are significant for human health and research. Through behavioral assays, we were able to confirm that Netrin4 does act as a chemorepellant. In addition, our ELISA and Western blots also helped substantiate the idea that Tetrahymena produce Netrin-4 for physiological functions, as they possess receptors for these proteins. The exact purposes of Netrin-4 for this organism is unknown up to this point, so further testing is needed to determine the cellular mechanisms with which Netrin-4 is involved.

Campus Venue

Stevens Student Center Lobby

Location

Cedarville, OH

Start Date

4-3-2019 11:00 AM

End Date

4-3-2019 2:00 PM

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Included in

Biochemistry Commons

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Apr 3rd, 11:00 AM Apr 3rd, 2:00 PM

Netrin-3 and Netrin-4-Like Proteins are Secreted from Tetrahymena thermophila

Cedarville, OH

Netrins are signaling proteins, acting as chemorepellants or chemoattractants, and their role is especially important in early growth in organisms. In studies involving Tetrahymena thermophila, netrin proteins often act as chemorepellants, so research centered around verifying if this was also true for Netrin-4 protein. Since Netrin-1 and Netrin-3 have been shown to influence neurological and developmental growth in organisms, the implications for discovering the cellular effects of Netrin-4 are significant for human health and research. Through behavioral assays, we were able to confirm that Netrin4 does act as a chemorepellant. In addition, our ELISA and Western blots also helped substantiate the idea that Tetrahymena produce Netrin-4 for physiological functions, as they possess receptors for these proteins. The exact purposes of Netrin-4 for this organism is unknown up to this point, so further testing is needed to determine the cellular mechanisms with which Netrin-4 is involved.

 

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