Type of Submission
Poster
Keywords
Tetrahymena Ras Wnt
Proposal
Cancer is a deadly disease which affects millions of people globally. Cancer ultimately arises from dysregulation of growth pathways, leading to loss of cell cycle control and tumor formation. Cancer cells maintain constant cell division, evade immune detection, ignore signals for apoptosis, and may acquire the ability to degrade the extracellular matrix and metastasize.
Tetrahymena thermophila are free-living (non-parasitic) ciliated unicellular eukaryotes belonging to Kingdom Protista. They are often used as a model system because they are relatively inexpensive and easy to culture and maintain. In addition, it is relatively simple to grow and harvest large numbers of cells for biochemical applications such as protein, DNA, or RNA purification. Because of their adaptability as a model system, we asked whether whether Tetrahymena possessed two common pathways often dysregulated in cancer; the Wnt signaling pathway and the Ras signaling pathway. In order to test our hypothesis, we used the Tetrahymena genome database to search for homologs of Wnt, Ras, and Raf. In addition, we used a Wnt agonist and an antagonist of Ras/Raf inhibition to determine whether the mitotic rate would be affected. While the Wnt agonist had no significant affect on mitosis, the Ras/Raf inhibitor significantly decreased mitotic rate in this organism. We also found homologs of Ras and Raf in the Tetrahymena genome database. Further studies are needed in order to obtain an accurate dose-response curve with the Ras/Raf inhibitor, BAY-293.
Creative Commons License
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Could a Protist help us understand cancer?
Cancer is a deadly disease which affects millions of people globally. Cancer ultimately arises from dysregulation of growth pathways, leading to loss of cell cycle control and tumor formation. Cancer cells maintain constant cell division, evade immune detection, ignore signals for apoptosis, and may acquire the ability to degrade the extracellular matrix and metastasize.
Tetrahymena thermophila are free-living (non-parasitic) ciliated unicellular eukaryotes belonging to Kingdom Protista. They are often used as a model system because they are relatively inexpensive and easy to culture and maintain. In addition, it is relatively simple to grow and harvest large numbers of cells for biochemical applications such as protein, DNA, or RNA purification. Because of their adaptability as a model system, we asked whether whether Tetrahymena possessed two common pathways often dysregulated in cancer; the Wnt signaling pathway and the Ras signaling pathway. In order to test our hypothesis, we used the Tetrahymena genome database to search for homologs of Wnt, Ras, and Raf. In addition, we used a Wnt agonist and an antagonist of Ras/Raf inhibition to determine whether the mitotic rate would be affected. While the Wnt agonist had no significant affect on mitosis, the Ras/Raf inhibitor significantly decreased mitotic rate in this organism. We also found homologs of Ras and Raf in the Tetrahymena genome database. Further studies are needed in order to obtain an accurate dose-response curve with the Ras/Raf inhibitor, BAY-293.
