Type of Submission
Poster
Keywords
Tetrahymena, mitotic regulation, H3K27, acetylation, gene regulation
Proposal
Tetrahymena thermophila have been used as a model system for decades, due in part to the fact that they are inexpensive and easy to grow. Cells can easily be grown in large quantities if protein or nucleic acid extraction is necessary. However, transfection of Tetrahymena thermophila with plasmids has traditionally been accomplished either by using electroporation or a gene gun, neither of which is particularly inexpensive or efficient. We sought to transfect Tetrahymena with the pCas9T plasmid using commercially available liposomes in order to present a simpler alternative to the classical transfection methods. Both DNA electrophoresis and RNA sequencing indicates that our plasmid is entering the cell.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Publication Date
2024
Successful Transfection of the pC9T Plasmid into Tetrahymena Thermophila Using Liposomes
Tetrahymena thermophila have been used as a model system for decades, due in part to the fact that they are inexpensive and easy to grow. Cells can easily be grown in large quantities if protein or nucleic acid extraction is necessary. However, transfection of Tetrahymena thermophila with plasmids has traditionally been accomplished either by using electroporation or a gene gun, neither of which is particularly inexpensive or efficient. We sought to transfect Tetrahymena with the pCas9T plasmid using commercially available liposomes in order to present a simpler alternative to the classical transfection methods. Both DNA electrophoresis and RNA sequencing indicates that our plasmid is entering the cell.
