Pharmaceutical Sciences Faculty Publications
Enhanced Detection of Sphingoid Bases Via Divalent Ruthenium Bipyridine Complex Derivatization and Electrospray Ionization Tandem Mass Spectrometry
Document Type
Article
Publication Date
1-1-2009
Journal Title
Analytical Chemistry
ISSN
1520-6882
Volume
81
Issue
1
First Page
495
Last Page
502
DOI
10.1021/ac8019043
PubMed ID
19055420
PubMed Central® ID
PMC3006443
Abstract
Sphingoid bases, such as unsaturated sphingosine (So) and its corresponding dihydro-saturated species sphinganine (Sa), are present in cell samples in low abundance. This fact combined with their low-to-moderate electrospray ionization (ESI) potential, compared to other sphingolipids such as sphingomyelins, limits their detection and quantitation by liquid chromatography-tandem mass spectrometry (LC-MS(2)). To enhance the ESI efficiency of sphingoid bases, a novel procedure to generate stably derivatized analytes that enhance the LC-MS(2) detection of sphingoid bases when analyzed using LC-MS(2) was developed. In this method, a ruthenium complex, [4-(N-succimidyloxycarbonyl propyl)-4'-methyl-2,2'-bipyridine] bis(2,2'-bipyridine) Ru(II) dihexafluorophosphate, is added directly to a cell extract. This complex reacts with and covalently binds to an amino group within the sphingoid bases. The dicationic nature of the ruthenium ion enhances the compound's ionization efficiency resulting in increased LC-MS(2) signals for the derivatized sphingoid bases. Consequently, the detection and quantitation of sphingoid bases are greatly improved.
Keywords
Chromatography, liquid, organometallic compounds, ruthenium, spectrometry, mass, electrospray ionization, sphingolipids, sphingosine, tandem mass spectrometry
Recommended Citation
Masood, M. Athar; Xu, Xia; Acharya, Jairaj K.; Veenstra, Timothy D.; and Blonder, Josip, "Enhanced Detection of Sphingoid Bases Via Divalent Ruthenium Bipyridine Complex Derivatization and Electrospray Ionization Tandem Mass Spectrometry" (2009). Pharmaceutical Sciences Faculty Publications. 299.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/299