Pharmaceutical Sciences Faculty Publications
A Mutation in the SH2 domain of STAT2 prolongs tyrosine phosphorylation of STAT1 and promotes type I IFN-induced apoptosis.
Document Type
Article
Publication Date
7-1-2007
Journal Title
Molecular Biology of the Cell
ISSN
1059-1524
Volume
18
Issue
7
First Page
2455
Last Page
2462
DOI
10.1091/mbc.e06-09-0843
PubMed ID
17442890
PubMed Central® ID
PMC1924825
Abstract
Type I interferons (IFN-alpha/beta) induce apoptosis in certain tumor cell lines but not others. Here we describe a mutation in STAT2 that confers an apoptotic effect in tumor cells in response to type I IFNs. This mutation was introduced in a conserved motif, PYTK, located in the STAT SH2 domain, which is shared by STAT1, STAT2, and STAT3. To test whether the tyrosine in this motif might be phosphorylated and affect signaling, Y631 of STAT2 was mutated to phenylalanine (Y631F). Although it was determined that Y631 was not phosphorylated, the Y631F mutation conferred sustained signaling and induction of IFN-stimulated genes. This prolonged IFN response was associated with sustained tyrosine phosphorylation of STAT1 and STAT2 and their mutual association as heterodimers, which resulted from resistance to dephosphorylation by the nuclear tyrosine phosphatase TcPTP. Finally, cells bearing the Y631F mutation in STAT2 underwent apoptosis after IFN-alpha stimulation compared with wild-type STAT2. Therefore, this mutation reveals that a prolonged response to IFN-alpha could account for one difference between tumor cell lines that undergo IFN-alpha-induced apoptosis compared with those that display an antiproliferative response but do not die.
Keywords
Amino acid motifs, apoptosis, cell line, tumor, cell nucleus, conserved sequence, interferon type I, janus kinases, mutation, phosphorylation, phosphotyrosine, transcription, genetic
Recommended Citation
Scarzello, Anthony J.; Romero-Weaver, Ana L.; Maher, Stephen G.; Veenstra, Timothy D.; Zhou, Ming; Qin, Angel; Donnelly, Raymond P.; Sheikh, Faruk; and Gamero, Ana M., "A Mutation in the SH2 domain of STAT2 prolongs tyrosine phosphorylation of STAT1 and promotes type I IFN-induced apoptosis." (2007). Pharmaceutical Sciences Faculty Publications. 373.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/373