Pharmaceutical Sciences Faculty Publications
Characterization of Microorganisms and Biomarker Development from Global ESI-MS/MS Analyses of Cell Lysates
Document Type
Article
Publication Date
6-1-2000
Journal Title
Analytical Chemistry
ISSN
0003-2700
Volume
72
Issue
11
First Page
2475
Last Page
2481
DOI
10.1021/AC991307N
PubMed ID
10857623
Abstract
The capability for sensitive and accurate identification of microorganisms has potential applications that include the monitoring of industrial bioprocessing operations, food safety analyses, disease diagnosis, and detection of potential biological hazards. Efforts based upon matrix-assisted laser desorption/ionization mass spectrometry to detect and identify specific microorganisms have been actively pursued for several years. We report a new method being developed to select useful biomarkers for the identification of microorganisms based upon electrospray ionization (ESI)-ion trap mass spectrometry. Crude cell lysates are processed using a recently developed dualmicrodialysis device and then directly infused into an ion trap MS. The low ESI flow rate and precursor ion accumulation capability of the ion trap MS enables high-sensitivity MS/MS analyses. Precursor ions are automatically selected and analyzed using tandem MS (MS/MS) to produce "global" MS/MS surveys and processed to yield two-dimensional MS/MS spectral displays. Such global MS/MS surveys are demonstrated for Escherichia coli lysates. The distinctive MS/MS spectral patterns can be used to identify mass spectrometric-detected species useful as biomarkers, which then provide a basis for confident microorganism identification. The results presented demonstrate the application of this method for the identification of microorganisms, as well as for detection of bacteriophage MS2 in the presence of a large excess of E. coli.
Keywords
Biomarkers, escherichia coli, levivirus, mass spectrometry, microdialysis
Recommended Citation
Xiang, Fan; Anderson, Gordon A.; Veenstra, Timothy D.; Lipton, Mary S.; and Smith, Richard D., "Characterization of Microorganisms and Biomarker Development from Global ESI-MS/MS Analyses of Cell Lysates" (2000). Pharmaceutical Sciences Faculty Publications. 521.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/521